Utilization of integrated Michaelis-Menten equation to determine kinetic constants
نویسندگان
چکیده
منابع مشابه
A Comparison of Estimates of Michaelis-menten Kinetic Constants from Various Linear Transformations.
The parameters which characterize this equation, and which must ordinarily be estimated from the observed data, are ‘v,,,, the maximum initial velocity which is theoretically attained when the enzyme has been “saturated” by an infinite concentration of substrate, and K,, the Michaelis constant which is numerically equal to the concentration of substrate for half-maximal initial velocity. Since ...
متن کاملA Comparison of Estimates of Michaelis-Menten Kinetic Constants from Various Linear Transformations
The parameters which characterize this equation, and which must ordinarily be estimated from the observed data, are ‘v,,,, the maximum initial velocity which is theoretically attained when the enzyme has been “saturated” by an infinite concentration of substrate, and K,, the Michaelis constant which is numerically equal to the concentration of substrate for half-maximal initial velocity. Since ...
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The summation of the effects of two or more reversible inhibitors of various types on the initial velocity of enzyme systems obeying Michaelis-Menten kinetics is described by the the general relation: (formula: see text) wherein v1,2,3...n is the velocity of reaction in the simultaneous presence of n inhibitors, vi is the velocity observed in the presence of each individual inhibitor, and v0 is...
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When the concentration dependence of an enzymic reaction or a transport process can be described by the sum of two Michaelis-Menten terms, reliable data that relate reaction rate and substrate concentration can be obtained even when as much as 70 per cent of substrate was consumed during the assay. Each data pair consists of the average reaction rate during an assay and the concentration where ...
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The Michaelis-Menten equation, a rectangular hyperbola with two ~arameters ~o b~ specified, . occurs prominently 1n enzyme k,netlcs." Often, ~n consulting situations we need to (i) obtain estlmates standard errors, and confidence intervals for the unknown parameters, which define this nonlinear model for one set of experimental conditions and (ii) compare the parameter estimates under different...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2007
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.32